Evaluating the expression patterns of multiple inhibitory receptors associated with T-cell exhaustion using multicolor flow cytometry

BD Horizon Brilliant™ dyes

Persistent antigen stimulation of T-cells, typically observed during chronic infections or in tumor microenvironments, results in the loss of antigen-specific CD8+ T-cell effector function. This phenomenon is referred to as T-cell exhaustion and is characterised by the progressive loss of function and proliferative capacity, eventually resulting in clonal elimination.

A typical feature of T-cell exhaustion is the increased expression of inhibitory receptors, which attenuate T-cell activation through a variety of processes. Studies on the two well known inhibitory receptors, CTLA-4 and PD-1, have shown that their inhibition reverses T-cell exhaustion, diminishes tumor growth and improves survival rates. However, the inhibition of these two receptors has been seen to be ineffective in many patients due to the immune system developing additional, non-redundant inhibitory mechanisms.

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Reformulated for decreased spillover:
the new BD Horizon Brilliant™ Ultraviolet dyes.

BD is committed to developing a reagent portfolio that allows you to fully optimize your multicolor flow experiments. To this extent the BD Horizon Brilliant™ Ultraviolet (BUV) tandem dyes have been reformulated. The spectra of the new and original BUV tandem dyes are very similar so the existing laser line and filter sets are still optimal for excitation and detection. The new reagents exhibit similar or brighter signals compared to the previous reagents. There may be some spillover differences, so we recommend resetting compensation values when using the new version of the dye. Most notably, the spillover into the BD Horizon BUV395 channel will be reduced.


BD Horizon Brilliant™ dyes

BD Horizon Brilliant™ dyes
These unique polymer dyes are typically brighter than conventional dyes—a breakthrough in this field. 


BD Horizon Brilliant™ Blue dyes

Optimally resolve dim populations with brighter options for the blue laser. BD Horizon Brilliant™ Blue 515 (BB515) is brighter than FITC, and BD Horizon Brilliant™ Blue 700 (BB700) is a brighter alternative to PerCP-Cy™5.5.

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