BD Cell and Biomarker Preservation Solutions


Ambitious research begins with valuable samples..

Research relies on the principle of consistency and the reliability of methods to gain valuable insights. Obtaining samples for research outside of the laboratory increases the variation factor and may provide inconsistent results. These factors are attributed to pre-analytical variables and account for 62% of errors in the clinical environment1. In areas of immunology, transcriptomics, and others alike, non-specific blood collection tubes, time & temperature, and variation in handling of samples can adversely impact the quality and consistency of data. The correct collection, transportation, and storage of blood samples are the first important steps in research and clinical breakthroughs.


Unlocking the key biomarkers in blood requires thought and care. BD Vacutainer® has over 70 years of blood specimen management experience to share.

Category Biomarker Application* Biomarker stabilisation
Disease Biomarker Analysis Circulating cell-free DNA (ccfDNA) – Early detection of tumors, transplant rejection2, predicting / monitoring therapy effectiveness in cancers through liquid biopsies3 PAXgene® Blood ccfDNA Tube for the stabilisation of ccfDNA from whole blood
Genomic Analysis DNA – Diagnosing inherited diseases, susceptibility to disease, predicting therapy effectiveness4 PAXgene® Blood DNA Tube for the stabilisation of DNA from whole blood
Transcriptomic Analysis Cellular RNA – Susceptibility to disease, early detection, predicting / monitoring therapy effectiveness such as myeloid leukaemia, cardiovascular diseases, breast cancer, and research in virus mutation5 PAXgene® Blood RNA Tube for the stabilisation of cellular RNA from whole blood
Proteomic Analysis Proteins – Susceptibility to early detection, predicting / monitoring therapy effectiveness, and disease such as diabetes6, metabolic and obesity7 research BD® P100 & P800 for the stabilisation of Proteomics and Metabolic Peptides from whole blood
Cell Analysis Peripheral Blood Mononuclear Cells (PBMCs) – Immunology and vaccine research & development, and transplant compatibility8 BD Cell Preparation Tube (CPT™) for the stabilisation of Peripheral Blood Mononuclear Cells
Infectious Disease Infectious DNA / RNA – Detection of infectious diseases (e.g. HIV9, HCV10) and monitoring treatment effectiveness BD Plasma Preparation Tube (PPT™) for the stabilisation of Infectious DNA / RNA
 

*These are examples of applications where BD and PAXgene® products can be utilized for research using cell and biomarker preservation tubes for whole blood. Users must validate use of product for their specific molecular diagnostic assay.

BD - The trusted name in blood specimen management

For over 70 years, BD has been a trusted name for blood specimen management solutions. The BD Vacutainer® family of products for blood cell and biomarker preservation are widely used in clinical and biomarker research across the world, in conjunction with a variety of downstream molecular, proteomics and cellular applications. These products can help ensure consistency in results, accuracy of data and workflow efficiency when measuring biomarkers.

Nucleic acids Immunology Proteomics Viral loading
 

Mean RNA integrity number (RIN) scores for all specimens in total (mean) are shown for the indicated storage times. The error bars indicate the standard deviations of RIN scores of all specimens from all subjects**.

PAXgene® Blood RNA Tubes

  • Coupled with the PAXgene® Blood RNA Kit (IVD), constitutes the FDA-cleared, CE-marked PAXgene® Blood RNA System
  • Single tube for collection, transport, and storage
  • Stabilises intracellular RNA
  • Specimen transport and storage at room, refrigerated or frozen temperatures*
  • Stabilisation:
    • 15-25°C for up to 3 days
    • 2-8°C for up to 5 days11
    • -20°C or -70°C for 11 years and long-term study still ongoing**

The PAXgene® Blood RNA Tube is CE marked for in vitro diagnostic use

*Specimen needs to be stored upright at room temp for a minimum of 2 hours before transferring to the fridge. Please follow IFU.
**For further details on long term storage and stability are available on preanalytix.com - link

Product Info:

Cat. No.
tube size
blood draw volume
762165 (CE IVD)
16x100 mm
2.5 mL
 


 

Minimized Blood Cell Lysis and Release of Genomic DNA. DNA profile of blood after extended storage in a PAXgene® Blood ccfDNA Tube (CE-IVD) is similar to blood collected in an EDTA Tube processed immediately12

PAXgene® Blood ccfDNA Tubes

  • Single tube collection, stabilisation, transport and storage
  • Formaldehyde-free, non-crosslinking stabilisation of circulating, cell-free DNA (ccfDNA)
  • Stabilisation:
    • 2 - 37°C for up to 3 days
    • 2 - 30°C for up to 7 days
    • 2 - 25°C for up to 10 days
    • Do not store blood-filled tubes below 2°C.

The PAXgene® Blood ccfDNA Tube is CE marked for in vitro diagnostic use

Product Info:

Cat. No.
tube size
blood draw volume
768165 (CE IVD)
16x100 mm
10.0 mL
 


 
 

Samples collected in PAXgene® Blood DNA Tubes (IVD) were processed at one site (internal) on the QIAsymphony. Median DNA yield was 28.9 µg DNA/ml blood, with 95% of samples ≥18.2 µg DNA/ml blood. DNA purity is equally high, with an A260/A280 ratio of 1.7–1.913.

PAXgene® Blood DNA Tubes

  • Compatible with automated purification technologies*
  • Validated for various collection and storage condition*
  • Unique blue cap and label identify tube for DNA testing
  • 2D barcode with tube ID, lot number and expiration date
  • Stabilisation:
    • 14 days at room temperature (18–25°C)
    • 28 days at 2–8°C
    • 3 days at 35°C
    • 52 weeks at –20°C and long-term study still ongoing**
    • 3 freeze-thaw cycles

The PAXgene® Blood DNA Tube is CE marked for in vitro diagnostic use

*Further details on purification technologies, long term storage and stability are available on prenalytix.com - link
**Further details available on preanalytix - link

Product Info:

Cat. No.
tube size
blood draw volume
761165 (CE IVD)
13x75 mm
2.5 mL
 

 

Supporting the pandemic research

The following studies demonstrate the utility of PAXgene® Blood RNA and BD CPT™ products for research in the fight against the pandemic.

RNA & Cellular Analysis :
1. Longitudinal Multi-omics Analyses - See more

RNA Analysis:
2. Whole-blood transcriptomic host response to the pandemic virus - See more
3. Multidimensional precision medicine transcriptional workflow - See more
4. Differential microRNA expression - See more

Cell Analysis
5. Early marker for severe pandemic cases: Neutrophil-to-VD2 T-cell ratio - See more

 

Blood transcriptome profiling and response of pandemic patients

The research activities in virology and transcriptomics have taken centre stage in the light of the global pandemic. Vaccine development and monitoring of the pandemic virus variants have become urgent priorities.

The PAXgene® RNA Blood Collection Tube provides a robust proprietary reagent that immediately stabilises RNA, with continued evidence to show that the integrity of RNA is not compromised even if stored frozen* for a least 11 years. > Link to product

The BD Vacutainer® Cell Preparation Tube (CPT) is designed to simplify PBMC extraction, remove the variability due to manual gradient techniques, reduce consumables, and ensure that sampling is consistent for challenging collection environments. > Link to product

*-20C and -70C

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